Fig. 4

CaMKIIα^T286A-CaM association during sLTP induction. a Averaged change in CaMKIIα^T286A-CaM association in a stimulated spine (green; n = 18 spines/4 neurons) in response to a single glutamate uncaging pulse (black square). The orange curve on CaMKIIα^T286A is obtained by curve fitting of an exponential function: B(t) = B₀·e^−t/τ. The dissociation time constant is obtained as τ = 1.2 ± 0.1 s. The data and fitted curve for CaMKIIα^WT are from Fig. 3c for the comparison. b Averaged change in CaMKIIα^T286A-CaM association in stimulated spines (green; n = 24 spines/7 neurons) during glutamate uncaging at 0.49 Hz. The orange curve indicates the decay of binding fraction change obtained by curve fitting of a double-exponential function: B(t) = B₀ [P_fast·exp(–t/τ_fast) + P_slow·exp(–t/τ_slow)]. The time constants for CaMKIIα^T286A are obtained as τ_fast = 1.0 ± 0.2 s (P_fast = 63%) and τ_slow = 356 ± 221 s (P_slow = 37%). The data and fitted curve for CaMKIIα^WT (black) are from Fig. 2d for the comparison. c Expanded view of the initial phase (left) and the late (right) phase of plot in b. All data are shown in mean ± sem, and sem of time constants is obtained by bootstrapping