Fig. 3
Ifb-Fwd and Ifb-Bwd mediate intersegmental feedback signaling. a Temporally stacked images of dual-color calcium imaging (pseudocolored such that Ifb-Fwd>GCaMP is green and A02e>RGECO is blue). b An example of calcium imaging of Ifb-Fwd (green) and A02e (blue). Ifb-Fwd is active during forward (f) but not backward (b) fictive locomotion (64 forward waves and 8 backward waves from 8 larvae). A02e activity was used to monitor wave propagation. c Correlation coefficients of calcium signals between Ifb-Fwd and A02e across trials in forward and backward waves. Each horizontal line corresponds to a single trial. d Summary of the activity and connectivity of Ifb-Fwd. e Temporally stacked images of dual-color calcium imaging (pseudocolored such that Ifb-Bwd>GCaMP is magenta and A02e>RGECO is blue). f An example of calcium imaging of Ifb-Bwd (magenta) and A02e (blue). Ifb-Bwd is active during backward (b) but not forward (f) fictive locomotion (10 forward waves and 19 backward waves from three larvae). g Correlation coefficients of calcium signals between Ifb-Bwd and A02e across trials in forward and backward waves. Each horizontal line corresponds to a single trial. h Summary of the activity and connectivity of Ifb-Bwd. Scale bar, 40 µm (a, e)
