Fig. 7
Ifb-Fwd and Ifb-Bwd are required for the contraction of transverse muscles. a–d Muscle imaging using mhc-GFP during forward (a, b) and backward propagation (c, d). a, c The lengths of transverse muscles M23 are plotted. b, d Images of body wall muscles during wave propagations. White arrows indicate the contraction of the transverse muscle. Note the reduction in the contraction of transverse muscles during forward waves when the activity of Ifb-Fwd is blocked (a, b) and the reduction in the muscle contraction during backward waves when the activity of Ifb-Bwd is blocked (c, d). e Quantification of the contraction of transverse muscles. Data size: 10–18 waves from 4 to 6 larvae for each group. ***p < 0.005 (Ifb-Fwd > Kir: p = 5.6 × 10−9; Ifb-Fwd > Kir: p = 0.0031 by the two-sided Student’s t-test with the Bonferroni correction). See Methods section for statistical analysis. Center line, median; box limits, upper and lower quartiles; whiskers, 1.5× interquartile range; points, outliers. Scale bar, 200 µm (b, d)
