Fig. 9
Developmental vascular regulatory pathways are reactivated during neovascularisation in the less regenerative P7 neonatal heart after MI. a–c Permanent ligation of the left anterior descending coronary artery was performed on transgenic P7 hearts to mimic MI. Representative sections through P9 HLX-3:lacZ (a, n = 6 biologically independent animals), Dll4-12:lacZ (b, n = 5 biologically independent animals) and Ephb4-2:lacZ (c, n = 4 biologically independent animals) transgenic hearts collected two days after MI surgery show robust enhancer activity in ECs surrounding the injured tissue. Dashed grey lines indicate the injured myocardium, and boxed regions show magnified views within the injury (box 1), at the injury border zone (box 2), and at a remote region (box 3). Sections through age-matched control hearts are shown alongside, with comparable boxed regions selected for the magnified views. lv, left ventricle; rv, right ventricle. Grey scale bars represent 200 µm
