Fig. 1
From: IL-33 drives group 2 innate lymphoid cell-mediated protection during Clostridium difficile infection
IL-33 is upregulated by host in response to C. difficile infection. Mice were infected with R20291 (CDT+) or attenuated R20291_cdtb (CDT-) and whole-cecal tissue transcriptomic analyses was performed on day 3 post infection. a Heatmap of genes upregulated (red) or downregulated (blue) in response to CDT toxin. b Volcano plot highlighting IL-33 (blue) among genes altered (>0.5 logFC = red). c ELISA of IL-33 protein within the cecal tissue (day 3) of R20291 infected, R20291_cdtb infected, or uninfected antibiotic-treated (ABX) mice. d Enriched pathways of the top upregulated transcripts (log FC > 0.5; p < 0.05) listed by significance (−log10(p)) and created using the ConsensusPathDP database. (a, b, n = 5 biologically independent animals). c Comparison made by ANOVA (n = 13, 13, 20 combined from two independent experiments). Individual n numbers in each panel are shown in same order as their corresponding groups from left to right, and this arrangement is applied to other relevant figures in the text. Statistical significance is demarked as *P < 0.05, **P < 0.01, and ***P < 0.001, throughout the text. Error bar indicates SEM
