Fig. 3
Single cell Venus::HES5 dynamics correlate with cell state. a Example single cell tracks for each cluster from 1 representative experiment. ‘*’ denotes cell division. Dorso-ventral axis in μm from floorplate. b Single cell tracks (n = 54) over 12-h in a single E10.5 spinal cord slice movie. Black dotted line—ventricle. Grey—ventricular zone and green—mantle zone measured by Sox2/NeuN immunostaining of two ex vivo slices. c Average distance of single cell from ventricle over 12-h track. Cells from three experiments clustered separately. Kruskall–Wallis with Dunn’s multiple comparisons test indicated cluster 1 vs. 3 adjusted p-value = 0.0014 (**). d Percentage of any cells found in 25 μm windows from ventricle in each cluster. Two-way ANOVA with Tukey multiple comparison test shows no difference between clusters <50 μm from ventricle. 76–100 μm from ventricle cluster 3 vs. 1 p < 0.0001, 3 vs. 2 p = 0.0009, 3 vs. 4 p = 0.014. e Displacement of cells in each cluster. Dot—average start position, arrow—average finish position. f Total distance travelled of single cells. Line is mean with SD. Kruskal–Wallis test with Dunn’s multiple comparison test shows cluster 1 vs. 3 adjusted p = 0.003 (**), cluster 3 vs. 4 adjusted p = 0.0017 (**). g Percentage of cells per cluster undergoing 0 or 1 divisions in 12 h. Chi-squared test of frequency data p = 0.0002. 181 cells, three experiments clustered separately. Cluster 1—black (n = 27 cells), cluster 2—sky blue (n = 33 cells), cluster 3—green (n = 67 cells), cluster 4—pink (n = 54 cells). Error bars—SD. Source data are provided in a Source Data file
