Fig. 4
Notch inhibition enriches for cluster 4-type Venus::HES5 dynamics. a Relative Venus::HES5 intensity of E10.5 ex vivo slices cultured in control DMSO (black) or 2 μM DBZ (pink). Error bars—SD (three experiments). Endpoint intensity—Mann–Whitney two-tailed test p = 0.0095 (**). b β-III tubulin (early neuronal marker) staining of DMSO or 2 μM DBZ-treated E10.5 ex vivo slices. fp floorplate. Scale bar 70 μm. c Displacement of single cells away from ventricle in apico-basal axis in control DMSO or 2 μM DBZ-treated E10.5 slices. Bold line—average, dashed line—SD from three experiments. Two-tailed t-test p < 0.0001 (****). d Hierarchical clustering of standardized single-cell Venus::HES5 expression in DMSO and 2 μM DBZ-treated E10.5 ex vivo slices. Columns show standardised individual cell Venus::HES5 expression dynamics in a heatmap. Rows represent time points after start of individual cell tracking. Cells tracked for 12-h time window with 15-min frame intervals. 295 cells, three experiments clustered together. Cluster labels defined using clustering of DMSO alone. Pink—DMSO cells, green—DBZ cells. e Percentage of cells in each cluster in DMSO and 2 μM DBZ-treated E10.5 ex vivo slices. Frequency data subject to Chi-squared test showed p < 0.0001 (****). DMSO n = 100 cells, 2 μM DBZ n = 195 cells, three experiments. Source data are provided in a Source Data file
