Fig. 2
CRATES can be used to efficiently generate a library of assembled CRISPR arrays in one pot. a Cloning scheme for the CRISPR array library. For this demonstration, a three-spacer array was generated, where each spacer could be one of five unique sequences. The resulting library contains 125 members. Note that the protocol for CRATES was slightly modified to further improve the cloning efficiency—see Methods. b Two independent libraries were generated followed by counting the number of green and white colonies. Nineteen white colonies from run #2 were then screened by colony PCR. All but one colony gave the expected band size of 528 bp. c Distribution of array abundances within the library from run #2. The distribution was assessed by next-generation sequencing. d Abundances of individual arrays within the 125-member library. The relative abundances are derived from next-generation sequencing results. A value of 1 represents the expected abundance if every library member is equally abundant. Source data are provided as a Source Data file
