Fig. 5
RNA-seq analysis revealed ranging crRNA abundances and an extraneous crRNA. RNA-seq analysis of the seven-spacer array from Fig. 1c co-expressed with FnCas12a in TXTL (top) or in HEK293T cells (bottom). The assembled array and FnCas12a were expressed followed by small-RNA isolation and RNA-seq analysis. Reads of no more than 50 nts were mapped to the expression construct for the synthetic FnCas12a array with seven spacers. The asterisk indicates the extraneous crRNA derived from the terminal repeat. For the experiments in HEK293T cells, the array was expressed from a CMV promoter
