Fig. 1
From: Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin
Retinal isomerization kinetics in purple membrane and bacteriorhodopsin (bR) microcrystals. a Schematic all-trans and 13-cis retinal covalently bound to Lys216 as a protonated Schiff base. b, c Direct comparison of kinetic traces in the ultraviolet/visible region for purple membrane (PM, blue) in H2O, pH = 5.6, and the bR microcrystals in lipidic cubic phase (red) as a function of peak intensities, 35 and 88 GW cm−2, respectively. Within the present signal-to-noise ratio, the dynamics of 13-cis isomer formation (PP, probe wavelength 670 ± 5nm, solid lines) and excited-state decay (excited-state absorption (ESA), 480 ± 5 nm, dashed) are identical. See Supplementary Fig. 5 for a complete comparison as a function of excitation density. Source data are provided for Fig. 1b, c as a Source Data file
