Fig. 1
Light- and 1O2-dependent EX1 degradation. Continuous light (CL)-grown 5-day-(d)-old transgenic seedlings of a ex1 and b ex1 flu expressing EX1-GFP under the control of the 35S promoter were transferred to the dark (for 2, 4, 8 h) and the 8-h-dark (D)-treated seedlings were re-exposed to light (for 0.5, 1, 2 h) at the light intensity of 100 µmolm-2 s-1. Total protein was extracted and analyzed by western blot. Chlorophyll a/b binding protein CP29 (Lhcb4) and cytosolic UDP-glucose pyrophosphorylase (UGP) were used as controls. EX1-GFP, Lhcb4, and UGPase were detected using antibodies against GFP, Lhcb4, and UGP, respectively. c The levels of EX1-GFP in the dark or after re-exposing to light were compared to its abundances under CL conditions. Average intensity values of the protein bands were calculated using AzureSpot software v14.0 (AZURE). Data represents the mean of three biological repeats. Error bars show standard error of the mean. Asterisks in c indicate statistically significant differences to CL condition (P < 0.05, Student’s t-test)
