Table 1 Trp oxidation in EX1 and associated PSII proteins

From: Oxidative post-translational modification of EXECUTER1 is required for singlet oxygen sensing in plastids

Protein

Sequence

AA modified

Observed mass (m/z)

Actual mass (Da)

Charge (z)

Error (ppm)

Prob. (%)

EX1

643RWoxVDGELVILDGK654

Trp643

688.36

1374.71

2+

4.7

100

D1

9ESESLWoxGR16

Trp14

498.23

994.44

2+

2.6

99

 

313VINTWoxADIINR323

Trp317

673.86

1345.70

2+

2.9

100

D2

14DLFDIMDDWoxLR24

Trp22

735.84

1469.66

2+

7.3

100

 

328AWoxMAAQDQPHENLIFPEEVLPRG349

Trp329

875.09

2622.26

3+

2.3

100

CP43

363APWoxLEPLR 370

Trp365

507.28

1012.54

2+

7.1

96

 

383DIQPWoxQER390

Trp387

552.26

1102.51

2+

2.1

96

CP47

273YQWoxDQGYFQQEIYR286

Trp275

978.44

1954.86

2+

6.5

100

 

288VSAGLAENQSLSEAWoxAK304

Trp302

896.95

2687.83

3+

180

99

  1. MS-based PTM analysis identified all three Trp oxidation variants, but the parameters for the double oxidation-induced NFK modifications with a mass shift of  + 32, have been shown here
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