Fig. 5

CB⁺ neurons increase in the PL throughout life. a DCX⁺ (arrowheads) and CB⁺ (arrows) cells in the MPL from birth to 67 years. (Inset) High magnification. b DCX⁺ (arrowheads) and CB⁺ (arrows) cells in the LPL from birth to 77 years. (Right) High magnification. c–e Quantification of cells in the LPL from birth to 77 years of age. Each data point corresponds to one individual; line indicates non-linear exponential fit. c Density of DAPI+ nuclei in the LPL from birth to 77 years. d CB⁺ cell density in the LPL from birth to 77 years. e Percentage of CB⁺DAPI⁺ cells in the LPL from birth to 77 years. f (Top) 77-year-old amygdala with two LPL cross-sections: one in the BLA containing few DCX+ cells and many CB+ cells and one in the white matter (wm) ventral to the BLA with many DCX+ cells and few CB+ cells. (Inset) higher magnification of the LPL in the white matter. (Bottom) Higher magnification of the LPL in the BLA and examples of small DCX+ cells in this region. g CB⁺TBR1⁺VGLUT2⁺ cells (arrows) in the (Left) MPL and (Right) LPL at 13 years. h DCX⁺ and TBR1⁺ cells in the MPL and LPL at 13 years. (Inset) Double-positive DCX⁺TBR1⁺ cell in the MPL. i DCX⁺TBR1⁺ and TBR1⁺NeuN⁺ neurons in the MPL at 13 years. (Top inset) small DCX⁺TBR1⁺ cell (arrow) and small DCX⁺TBR1^– cell (arrowhead). (Middle inset) intermediate-sized DCX⁺TBR1⁺ cell (arrow) and DCX^–TBR1⁺NeuN⁺ cell (arrowhead). (Bottom inset) Large DCX^–TBR1⁺NeuN⁺ cells (arrowheads). j DCX⁺TBR1⁺VGLUT2^– cells (arrows) and DCX^–TBR1⁺VGLUT2⁺ cells (arrowheads) in the MPL at 13 years. Scale bars: 100 µm (a, b left, f top, h), 20 µm (a insets, b right, f top inset, f bottom left, g, i left, j), 10 µm (h left inset, i right insets), 5 µm (f lower right insets)