Fig. 4
From: Transcription factor YcjW controls the emergency H2S production in E. coli
Deletion of pspE in ΔmstA-sup decreases H2S biosynthesis during exponential growth. a qRT-PCR of three thiosulfate sulfurtransferase genes. RNA was isolated from cells grown to OD600 ~ 0.4. Only pspE expression was significantly increased in both ΔmstA-sup and ΔycjW. Values are means ± SD (n = 3). *p < 0.05 as determined by the Student's t test. b H2S levels in ΔmstA-sup; ΔpspE were undetectable till OD600 reached 0.8. Values are means ± SD (n = 3). *p < 0.05 as determined by the Student's t test. c ΔmstA-sup/ΔpspE had increased sensitivity to gentamicin compared with WT and ΔmstA-sup. Overexpression of pspE in ΔmstA increased tolerance to gentamicin compared with ΔmstA. Values are means ± SD (n = 3). d In cells lacking MstA which is responsible for H2S production, S258N YcjW upregulates PspE as an alternate route of H2S biosynthesis. Source data are provided as a Source Data file
