Fig. 1
Timing of cycle exit in GCPs. a GCPs exit the cell cycle within 24 h of plating as judged by PI-FACS analysis. FACS analysis was performed on GCPs purified from P6 mice and plated for the time intervals indicated. FlowJo software was then used to assess the percentage of cells in the G1, S, or G2/M phase. b Purified GCPs exit the cell cycle within 24 h of plating as judged by EdU incorporation studies. GCPs purified from P6 mice were processed for EdU incorporation, which was normalized to the total number of cells labeled with Hoechst staining. c Purified GCPs from P6 mice were plated and processed for the RNA expression at the time intervals indicated for the proliferative markers, Ccnb1 and Atoh1, and the differentiation markers, Tuj1 and Gap43. qRT-PCR was performed and normalized to Gapdh. d Short-time series modeling of mRNAs during cell-cycle exit. Plots represent mRNA expression profiles during GCP cell-cycle exit. Representative clusters (#47, #75 and their related cellular processes) are shown. The red line represents the consensus for each cluster. The gray lines represent individual mRNA expression profiles. The associated biological processes as defined by DAVID41 in each cluster are shown. The identity of each gene in the cluster can be found in Supplementary Data 1. The results are shown as the average values of three independent experiments and are represented as the mean ± SEM. A one-way ANOVA followed by Bonferroni multiple comparison testing was performed (*p < 0.05, **p < 0.01). Source data can be found in source data graphs under tabs for a–c
