Table 1 Comparisons of TRFS-green, TRFS-red and Fast-TRFS
Properties | Previous work | This work | |
|---|---|---|---|
TRFS-green | TRFS-red | Fast-TRFS | |
Response rate (TCEP, 1 mM) | + (>2 h) | ++ (>1 h) | +++ (<1 min) |
Response rate (TrxR, 50 nM) | + (>3 h) | ++ (>1.5 h) | +++ (~5 min) |
F/F0 (TCEP, 1 mM) | + (~30) | ++ (~90) | +++ (>150) |
Selectivity (TrxR/GSH) | ++ (15.6) | + (12.8) | +++ (55.7) |
Ex & Em (nm) | ++ (438/538) | +++ (615/661) | + (345/460) |
Sensing mechanism | Cleavage- CDR | Cleavage-CDR | Cleavage only |
