Fig. 3 | Nature Communications

Fig. 3

From: Monoallelic expression and epigenetic inheritance sustained by a Trypanosoma brucei variant surface glycoprotein exclusion complex

Fig. 3

VEX2 depletion results in multi-VSG expression. a Cumulative growth following tetracycline (Tet) induced VEX2-knockdown; *p < 0.05; **p < 0.01 (multiple t tests). Protein blotting (b), immunofluorescence analysis (c), and flow cytometry (d), to assess VSG expression following VEX2-knockdown (24 h). EF1α, loading-control in (b); in c, DNA was counter-stained with DAPI and the graph depicts the rapid increase of dual VSG expressing cells following VEX2 knockdown by microscopy analysis. Scale bars, 5 μm (left hand side) and 2 μm (right hand side). Percentages in d indicate double-positive cells. e Quantitative mass spectrometry analysis of surface-VSGs following VEX2-knockdown (48 h). The inset shows data for wild-type cells. emPAI, exponentially modified Protein Abundance Index. Error bars (not visible in a), SD; data are averages from two (a/c/e) independent biological replicates and representative of independent experiments (a–d). Source data are provided as a Source Data file

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