Fig. 5 | Nature Communications

Fig. 5

From: An anionic human protein mediates cationic liposome delivery of genome editing proteins into mammalian cells

Fig. 5

Delivery of zinc-finger nucleases (ZFNs) using ProTα. a Structure of ProTα-fused ZFN versus ZFN alone. b ProTα-fused ZFNs or unfused ZFNs targeting the AAVS1 site in HEK293T cells were delivered using Lipofectamine RNAiMAX. ProTα enables efficient delivery of ZFNs and induce indels at a mid-nanomolar concentrations. Both left and right ZFN components, as well as lipid, are required for efficient indel generation. Values and error bars represent the mean and standard deviation of three independent biological replicates performed on different days. Source data are available in the Source Data file

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