Table 1 Protein delivery using Lipofectamine RNAiMAX in HeLa-DsRed cell line

From: An anionic human protein mediates cationic liposome delivery of genome editing proteins into mammalian cells

Protein fused to Cre

Concentration at which 50% of cells are recombined (nM)

ProTα

1.1 ± 0.18

Polyadenylate-binding protein-interacting protein 2

290 ± 60

Troponin C

57 ± 19

DPH3 homolog

36 ± 5

RNA Polymerase II Subunit F

110 ± 58

Multiple coagulation factor deficiency protein 2

140 ± 170

ADP-ribosylation factor-like protein 2-binding protein

20 ± 9

NF-kappa-B inhibitor alpha

63 ± 7

DNA damage-inducible transcript 3 protein

89 ± 5

Carbonic anhydrase VIII

84 ± 22

Protein S100-B

47 ± 60

Sirtuin-1

49 ± 18

(–30)GFP

11 ± 3.0

No fusion

73 ± 12

  1. 12 human proteins were chosen as candidates for lipid nanoparticle delivery. EC50 is defined as the concentration at which 50 % of the cells contain red fluorescence
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