Fig. 4
From: Mutations in SMARCB1 and in other Coffin–Siris syndrome genes lead to various brain midline defects
Reduced neural stem and progenitor cells in the embryonic mutant brain. a Immunostainings of coronal forebrain sections from three mutant and three littermate control embryos at stage E14.5. b The thickness of the Sox2-, Tbr2-, and Ctip2-positive cortex layers is significantly reduced in the mutant compared to control animals. All bars display the mean with standard deviations. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 (unpaired two-tailed Student’s t test). c Cell densities within the Sox2-, Tbr2-, and Ctip2-positive layers of the mutant compared with the control cortex. p values (unpaired two-tailed Student’s t test): n.s. not significant. d Cells isolated from the mutant forebrain of E15.5 embryos fail to form large neurospheres in comparison with cultures established from E15.5 control brain tissue. Cultures of passage one (3 days after passaging) are shown. e Hes5 expression in coronal brains sections from E16.5 and E18.5 mutant and control animals detected by in situ hybridization (blue signals). Enlarged regions (framed areas) include the site of glial wedge development (arrowheads). Nuclear fast red stainings were performed to visualize the region of corpus callosum development, which appears white due to the paucity of nuclei. Scale bars: 100 µm (a, d), 200 µm (e). Source data are provided as a Source Data file
