Fig. 1
From: Toxin-mediated ribosome stalling reprograms the Mycobacterium tuberculosis proteome
MazF-mt9 targets only tRNALys43-UUU in vivo. a Histogram representing the ratio of cleavage by MazF-mt9 identified using 5′ RNA-seq at each nucleotide within the lysT gene (tRNALys43-UUU) in M. tuberculosis H37Rv after 7 days of toxin induction. Genomic positions and the negative strand sequence are shown. b Representation of the only MazF-mt9 target, tRNALys43-UUU (adapted from Schifano et al.15). Anticodon (UUU), in red; cleavage site, yellow arrow. c Northern analysis of RNA from ±MazF-mt9 M. tuberculosis cells using the tRNA isoacceptor-specific oligonucleotides indicated. Uncropped Northern blot images are provided as a tab within the Source Data file
