Fig. 3
From: Toxin-mediated ribosome stalling reprograms the Mycobacterium tuberculosis proteome
Ribo-seq confirms transcriptome-wide AAA Lys-specific ribosome stalling. a, b, e, f Ribo-seq read coverage in reads per million (rpm) of AAA Lys-containing M. smegmatis (a, b) or M. tuberculosis mc2 6206 (e, f) transcripts in ±MazF-mt9 cells (arrows indicate direction of translation). The location of in-frame AAA Lys codons and out-of-frame AAAs are indicated (red arrow/gray box and blue arrow head, respectively). Scale bars illustrate a 100 bp genomic distance. c, g, Average read counts (rpm) of mapped ribosomes surrounding AAA Lys codons in +MazF-mt9 (red line) or -MazF-mt9 (blue) cells; light shading represents the standard deviation. d, h Ratio of codon occupancy (MazF-mt9 induced vs. uninduced) at the predicted ribosomal A-site position identified in the mapped ribosomal footprints, grouped by amino acid or stop codon. Source data for d and h are provided in a separate tab within the Source Data file
