Fig. 5

The ensheathment capacity of myelinating SCs is independent of mTOR signaling. mTOR deletion in Fbxw7 mutant nerves failed to suppress aberrant SC-axon interactions. Apparent multi-axonal myelination and SCs that appeared to both myelinate and encompass multiple small non-myelinated axons were present in both Dhh^cre(+);Fbxw7^fl/+;mTOR^+/+ (b, e) and Dhh^cre(+);Fbxw7^fl/+;mTOR^fl/fl (HetΔmTOR) (c, f) nerves. Neither phenotype was ever observed in sibling controls (a, d; p < 0.0001(****); ANOVA). Tracing the BL (e, f; white arrows) suggested these behaviors were the acts of single SCs. Both phenotypes were also present in the sole Dhh^cre(+);Fbxw7^fl/fl;mTOR^fl/fl (cKOΔmTOR) animal we were able to collect (g, h). Asterisks above bars indicate comparisons with controls; comparisons between Dhh^cre(+);Fbxw7^fl/+ and Dhh^cre(+);Fbxw7^fl/+;mTOR^fl/fl were not significant. P3: N = 4 control, 6 Dhh^cre(+);Fbxw7^fl//+;mTOR^+/+, 5 Dhh^cre(+);Fbxw7^fl/+;mTOR^fl/fl, 1 Dhh^cre(+);Fbxw7^fl/fl;mTOR^fl/fl. m = myelinated axon; a = unmyelinated axon. Error bars depict S.D.