Fig. 3
IFN-β is essential for the resolution of E. coli pneumonia. Female C57BL/6 mice were injected intratracheally with 5*106 live E. coli with anti-IFN-β antibody (1 μg/20 g b.w.) or isotype control (IgG) or saline (“-“). At 24 or 48 h, lungs were removed without lavage and analyzed for E. coli content (a), lung dry-to-wet weight ratio (c) and tissue MPO activity (d). In separate groups of mice bronchoalveolar lavage fluid protein concentration (b), total leukocyte (e), neutrophil (f) and monocyte/macrophage numbers (g), the percentage of annexin-V-positive (apoptotic) PMN (h), and the percentage of macrophages containing apoptotic bodies (i) were determined. Results are means ± SEM (n = 6 mice per group for control and anti-IFN-β, or 4 for IgG). *P < 0.05, **P < 0.01 (Dunn’s multiple contrast hypothesis test). Source data are provided as a Source Data file
