Fig. 1
From: Structure of the substrate-engaged SecA-SecY protein translocation machine
Overall structure of the SecA-SecY-substrate complex a Components of the translocation complex. The protein sequences are shown schematically as bars in different colors. G. thermodenitrificans (Gt) SecE, purple; SecY, salmon; sfGFP, green; B. subtilis (Bs) SecA, blue; and Enhancer, tan. The proOmpA signal sequence is highlighted in green. Cysteines (C) used for cross-linking (double arrow) are shown in red. The interaction between sfGFP and Enhancer are indicated by three dashed lines. The positions of some residues are indicated. b Scheme of the assembled translocation complex. c Cryo-EM density map of the SecA-SecY-substrate complex, with density for the individual proteins colored as in (a) and (b). The lipid nanodisc is colored in gray. Interaction sites between SecA and the lipid surface are indicated by red arrowheads. d Ribbon model of the complex. Individual proteins are colored and labeled as in (a) and (b)
