Fig. 1
From: Molecular basis of egg coat cross-linking sheds light on ZP1-associated female infertility
ZP1 I390fs404X mutant protein does not affect the secretion of hZP2-4. a Domain architecture of wild-type and mutant hZP1. SP signal peptide, CFCS consensus furin cleavage site, TM transmembrane domain. Inverted tripods mark N-glycosylation sites, with the conserved N-glycan of the ZP-N1 domain coloured red. Domain boundaries are indicated, and the 15-residue C-terminal extension resulting from the frameshift mutation is highlighted in dark red. b Anti-5His immunoblots of medium (left; 2.4 mL per lane) and cell lysate (right; 0.3 mL per lane) samples from HEK293T cells expressing hZP1 constructs show that hZP1Mut is retained intracellularly (red arrow). NR non-reducing conditions, R reducing conditions. c Co-expression with human ZP2-4 increases secretion of wild-type hZP1, but does not rescue secretion of hZP1Mut (240 μL medium per lane). d The secretion levels of hZP2, hZP3 and hZP4 do not change upon co-expression with either hZP1 or hZP1Mut (240 μL medium per lane)
