Fig. 9
From: Molecular basis of egg coat cross-linking sheds light on ZP1-associated female infertility
ZP4-N1 forms a non-covalent homodimer in chicken, but not in human. a SEC analysis of purified hZP4-N1 and cZP4-N1 and non-reducing Coomassie-stained SDS-PAGE analysis of the corresponding peak fractions. Peaks are normalised to 500 mAU, and elution volumes of the cZP1-N1 homodimer and the cZP1-N1 C66A monomer are indicated. b, c Immunoblot analysis in reducing conditions of His-pull-down experiments of co-expressed hZP4-N1-His/Myc or cZP4-N1-His/Myc. Samples were PNGase F-treated before SDS-PAGE. FT flow-through, E elution
