Fig. 4
From: Structural basis of Q-dependent transcription antitermination
QI-RNAP interactions that mediate QI engagement. a The main portion of QI interacts with β' dock, while H2 inserts into the RNA exit channel and interacts with the zipper, lid, and ZBD. Cyan, QI; gray, β′. b H2, L1, and L2 form a ring-like structure inside the RNA exit channel. RNAP is shown as surface. ZBD is omitted for clarity. Dark gray, β; light gray, β'; cyan, QI; pink, RNA. c Interactions between H1 and β' dock. d Interactions between H2 and the zipper, lid, and ZBD. e Effects on antitermination of substitutions of H1 and H2 (mean ± SEM; 3 determinations). f Effects on Q engagement of substitutions of H1 and H2 (mean ± SEM; 4 determinations). Error bars represent mean ± SEM out of n = 3 experiments. Source data are provided as a Source Data file
