Fig. 1
From: Pooled library screening with multiplexed Cpf1 library
Functional genomics screenings with benchmark CRISPR libraries a In SpCas9 or AsCpf1-based mono-cistronic libraries, each gene has multiple guides dispersed in different lentivirus. In AsCpf1-based multiplexed library, each gene only has one guide array construct. However, each array contains multiple different guides targeting one gene. b Pooled library screen pipeline schematics. MOI (Multiplicity of infection) c, d The percentage of the active construct for different libraries at the 5% false positive rate across four time points (c), and the increased percentage of the active construct for different libraries at the screen endpoint with a different controlled false positive rate (d). Pink: Cas9-based mono-cistronic CRISPR library (Cas9). Yellow: AsCpf1-based mono-cistronic CRISPR library (AsCpf1-Mono). Purple: AsCpf1-based multiplexed CRISPR library (AsCpf1-Multi). Error bars are present as standard deviations (s.d.) of triplicate. e, f The construct-level precision-recall curves for CRISPR libraries (e), and the gene-level precision-recall curves for CRISPR libraries (f). Each curve represents one biological replicate, three replicates in total. Pink: Cas9-based mono-cistronic CRISPR library. Purple: AsCpf1-based multiplexed CRISPR library. Yellow: AsCpf1-based mono-cistronic CRISPR library. g The rmAUC curves of CRISPR libraries describing population dynamics. rmAUC (ratio of the modified area under the curve) is calculated by (AUCx-0.498)/(AUCend-0.498) x 100%. AUCx: the area under the curve of construct-wise precision-recall curves for time point X. AUCend: the area under the curve of construct-wise precision-recall curves of an endpoint. Pink: Cas9-based mono-cistronic CRISPR library. Yellow: AsCpf1-based mono-cistronic CRISPR library. Purple: AsCpf1-based multiplexed CRISPR library. Error bars are present as s.d. of triplicate. Source data are provided as a Source Data file
