Fig. 4

Potential driver genes related to BM. a Twenty-seven genes included (1) genes with more than 3 mutations in BM tissues; (2) genes with a significantly different mutation rate between our patients and TCGA patients; (3) genes with expression or mutations associated with the overall survival of patients in TCGA; (4) genes reported as significantly mutated genes in a previous study and with at least 2 mutations in BM tissues; (5) genes encoding voltage-gated sodium channel proteins with at least 2 mutations in BM tissues. Significantly mutated genes identified in a previous study are colored in light blue. The mutation rate in our cohort and TCGA data are listed on the right side and compared. The P value of the Cox regression model corrected for age, sex, and stage in survival analysis is listed on the left side. *P < 0.05; **P < 0.01. b In the TCGA data, the expression of IKZF1 was significantly associated with poor survival among CRC patients. c A recurrent mutation in SCN7A was identified in the BM tissues of two patients. d In the TCGA data, the expression of SCN2A was significantly associated with poor survival among CRC patients