Fig. 5
From: Involvement of cigarette smoke-induced epithelial cell ferroptosis in COPD pathogenesis
Involvement of ferroptosis in human COPD lung. a WB showing expression levels of GPx4 and β-actin in HBECs isolated from never smokers (n = 6) or non COPD smokers (n = 6) or COPD patients (n = 7). The bar chart shown represent the mean ± SEM. *P < 0.05, **P < 0.01 by one-way ANOVA followed by Tukey’s multiple comparisons test. The correlation analysis between GPx4 expression level and the percentages of FEV1/FVC (n = 19) in HBECs from never smokers, non-COPD smokers, and COPD patients. b IHC of GPx4 in never smoker, healthy smoker and COPD lung. Original magnification ×400. Bar = 100 µm. c WB showing expression levels of NCOA4 and β-actin in lung homogenates from never smokers (n = 5) or non COPD smokers (n−6) or COPD patients (n = 15). The bar chart shown represent the mean ± SEM. *P < 0.05, **P < 0.01 by one-way ANOVA followed by Tukey’s multiple comparisons test. The correlation analysis between NCOA4 expression level and the percentages of FEV1/FVC (n = 31) in human lung homogenates from never smokers (n = 10), non-COPD smokers (n = 6), and COPD patients (n = 15). d IHC of NCOA4 in human never smoker, healthy smoker and COPD lung. Original magnification ×400. Bar = 100 µm. e LC-MS analysis of PC and their oxidation products in never smokers, smoker non-COPD, and COPD lung homogenates. MS of precursor ion scanning of m/z 184 in the positive ion mode were analyzed. PC-OOH/PC (18:0/18:2), (18:1/18:2), (16:0/20:4) shown in upper panel. non-COPD, n = 6; COPD, n = 6. LC-MS analysis of PE and their oxidation products in never smokers, smoker non-COPD, and COPD lung homogenates. *P < 0.05 by student’s t-test. MS of neutral loss scanning of 141 Da in the positive ion mode were analyzed. PE-OOH/PE (18:0/18:2), (18:1/18:2), (16:0/20:4) were shown in lower panel. non-COPD, n = 6; COPD, n = 7. *P < 0.05 by by student’s t-test. f Perls’ DAB and 4-HNE staining in never smoker, healthy smoker and COPD lung tissues (original magnification, ×400). Bar = 100 µm. g Representative TEM images of bronchial epithelial cells in never smoker lung and COPD lung (Brinkman Index = 2100, FEV1% = 62.5%). Scale bars ;2 µm. Throughout, n = independent patients
