Fig. 8

IL6–IL6Rα soluble complex antagonizes TFF1-mediated suppression of STAT3 activation. a AGS cells were infected with control or TFF1 adenoviruses (5 MOI), transfected or not with GP130 expression plasmid (500 ng mL⁻¹) and stimulated or not with IL6 (100 ng mL⁻¹). AGS expressing TFF1 showed a decrease of p-STAT3 (Y705) after GP130 transfection and IL6 stimulation. b AGS cells were transfected or not with GP130 expression plasmid (500 ng mL⁻¹) and treated with recombinant TFF1 (400 ng mL⁻¹), then stimulated or not with IL6 (100 ng mL⁻¹). TFF1 treated cells showed a decrease of p-STAT3 (Y705) after GP130 transfection and IL6 stimulation. c AGS cells were infected with control or TFF1 adenoviruses (5 MOI) and treated or not with IL6–IL6Rα soluble complex (10 ng mL⁻¹). AGS-expressing TFF1 showed no effect of p-STAT3 (Y705) after IL6–IL6Rα treatment as compared to control. d AGS cells were treated or not with TFF1 recombinant protein (400 ng mL⁻¹) and, or IL6–IL6Rα soluble complex protein (10 ng mL⁻¹). AGS cells treated with TFF1 and IL6–IL6Rα showed no effect of p-STAT3 (Y705) as compared to control cells treated with IL6–IL6Rα alone. The relative intensity ratio p-STAT3/β-Actin were calculated by the Image-lab software from BioRad, and presented under each blot. The results are expressed as mean ± SEM of at least 3 independent experiments; ***P < 0.001 by two-tailed Student’s t test, and P > 0.05 is considered nonsignificant (ns)