Fig. 9
From: Activation of STAT3 signaling is mediated by TFF1 silencing in gastric neoplasia
Human gastric cancer tissues demonstrate nuclear localization of STAT3 and loss of TFF1. a–f Representative immunohistochemical (IHC) staining images of TFF1 (upper panels a and b) and STAT3 (lower panels c and d) using human gastric cancer tissue microarrays that contained 108 cancer samples with their matching adjacent histologically normal stomach, original magnification is at ×200 and insets are at ×400. e, f Summary graphs of the IHC staining of TFF1 (e) and nuclear STAT3 (f) results using box-and-whisker blots to depict the smallest value, lower quartile, median, upper quartile, and largest value, (+) indicate the mean. g Schematic cartoon representing the role of trefoil factor 1 (TFF1) in regulating inflammatory signaling in gastric epithelial cells. Gastric cancer is associated with inflammation mediated by the release of cytokines such IL6 and TNFα from stromal and epithelial cells inducing activation of STAT3 and NFκB transcriptional factors. Loss of TFF1 promotes IL-6-mediated IL6Rα-GP130 complex formation, recruitment and phosphorylation of JAK2, with subsequent phosphorylation and nuclear localization of STAT3 to activate STAT3 transcription targets. The role of TFF1 in regulating NFκB has been described before16
