Fig. 2
From: Apoptotic signalling targets the post-endocytic sorting machinery of the death receptor Fas/CD95
ENTR1 requires binding to PTPN13 to regulate Fas cell surface levels. a Flow cytometry analysis of surface abundance of Fas in HeLa cells upon PTPN13 knock-down. Shift of the peaks towards the right in all the samples as compared to the control indicates increased Alexa 488 intensity. b Quantification of the flow cytometry analysis of Fas receptor levels expressed as a percentage relative control siRNA transfected cells. Bar graphs represent the mean of the Alexa Fluor 488 nm median intensities (n = 3), three independent experiments, one way ANOVA. c Interaction of wild-type and mutant ENTR1 with endogenous PTPN13. HeLa cells were transfected with the indicated GFP-tagged ENTR1 constructs followed by GFP-trap and western blotting. d Flow cytometry analysis of Fas surface levels upon ENTR1 knock-down and rescue with aforementioned ENTR1 constructs. Bar graphs represent the mean of the Alexa Fluor 633 nm median intensities. Data was collected from three independent experiments (n = 3), One-way ANOVA was performed, **p < 0.01, error bars represent ± SEM
