Fig. 1
From: SHB1 and CCA1 interaction desensitizes light responses and enhances thermomorphogenesis
SHB1 and CCA1/LHY regulate PIF4 expression under red light. a PIF4 expression in 4-day-old Col, shb1, Ws, and shb1-D seedlings in the dark and under 15 μmol m−2 s−1 red light for 3 hr. * and ** indicate significance levels p < 0.05 and p < 0.01, respectively. ns indicates not significant by Student’s two-tailed heteroscedastic t tests in this figure and subsequent figures. PIF4 expression in Col and shb1 b or Ws and shb1-D c under continuous red light after entrained under 12-hr light and 12-hr dark for 7 days from two biological replicates. d PIF4 protein accumulation in Col, shb1, Ws, and shb1-D under 12-hr red light and 12-hr dark photoperiod, shown as representative images from three biological replicates. e PIF4 protein quantification normalized to actin with standard error bars. The control blot shows PIF4 accumulation in Ws, shb1-D and pif4 at ZT6. f PIF4 expression in Ws, cca1, lhy, and cca1 lhy in the dark and under 15 μmol m−2 s−1 red light. Expression of PIF4 in each sample was normalized to that of UBQ10, and data are presented as the means ± SE. Source data are provided as a Source Data file
