Fig. 2
From: Alternative splicing regulates stochastic NLRP3 activity
The splicing landscape of human NLRs. a Gene expression of all previously identified RI-type LRR encoding genes (Fig. 1d–f). b Sashimi plot of NLRP3 expressed in human monocyte-derived macrophages established from five healthy human blood donors created with MISO. Read frequency within exons is plotted as RPKM and exon spanning reads are labeled with the number of mapped reads. The NLRP3 gene structure is plotted above, with boxes indicating exonic regions and arrows within the intronic stretches indicating the reading directions. Short repetitive LRR exons are highlighted with a gray box. The genomic location is depicted below. c, d Sashimi plots as in b, focused on exons 4–5–6 and 6–7–8, respectively. MISO ψ (% spliced in) values (red bars in histogram) indicate the calculated frequency of exon inclusion. 95% confidence intervals are indicated as gray bars in the histogram. Ψ and CI values are listed as well numerically. See also Supplementary Fig. 2 and Supplementary Table 1. Source data are provided as a Source Data file
