Fig. 5
From: Reconstitution of recombinant human CCR4-NOT reveals molecular insights into regulated deadenylation
Motifs from multiple CCR4-NOT recruitment factors inhibit deadenylation. a–c Deadenylation assays with equimolar concentrations (50 nM) of the 7-mer-A20 substrate and the CCR4-NOTMINI (a), CCR4-NOTFULL (b), or CCR4-NOTΔ10:11 (c) complexes, respectively. 2.5 µM of either MBP (as a negative control) or MBP-tagged Bam CBMWT, Roquin-C or NOT4 CBM, respectively, were tested with the reconstituted complexes. All three motifs inhibited the deadenylation reaction of all three complexes but the extent of the inhibition was variable. The CCR4-NOT complexes lacking the NOT10:NOT11 heterodimer were strikingly more sensitive to the inhibition by the peptide motifs. Source data are provided as a Source Data file
