Table 1 Cryo-EM data collection/processing
From: Molecular mechanism of setron-mediated inhibition of full-length 5-HT3A receptor
5-HT3A-granisetron (EMDB-0469; PDB-6NP0) | |
|---|---|
Data collection and processing | |
Magnification | ×130,000 |
Voltage (kV) | 300 |
Electron exposure (e–/Å2) | 40 |
Defocus range (μm) | −1.0 to −2.5 |
Pixel size (Å) | 0.532 |
Symmetry imposed | C5 |
Initial particle images (no.) | 243,290 |
Final particle images (no.) | 46, 757 |
Map resolution (Å) | 2.92 |
FSC threshold | 0.143 |
Refinement | |
Initial model used (PDB code) | 6BE1 |
Map sharpening B-factor (Å2) | −50 |
Model composition | |
Non-hydrogen atoms | 16,891 |
Protein residues | 16,300 |
Ligands | 591 |
B factors (Å2) | |
Protein | 111.12 |
Ligand | 117.85 |
RMS deviations | |
Bond lengths (Å) | 0.008 |
Bond angles (°) | 1.072 |
Validation | |
MolProbity score | 1.51 (95th percentile) |
Clashscore | 3.63 (97th percentile) |
Poor rotamers (%) | 0.55 |
Ramachandran plot | |
Favored (%) | 94.83 |
Allowed (%) | 5.17 |
Disallowed (%) | 0.00 |
