Fig. 3

Generation of an LCSS for the ASCL1^High and NEUROD1^High NE-Lung cancer subtypes. a The volcano plot showing the indicated fold changes and P-values derived from t-test statistic. Proteins differentially present in the secretome between ASCL1^High and NEUROD1^High cells with an adjust P-value (FDR) < 0.05 are highlighted in pink and light blue, respectively. b The relative fold changes of the 65 significantly regulated secreted proteins in the secretome of 12 cell lines were grouped by unsupervised hierarchical clustering. c Transcriptomic clustering of 69 human SCLC tumors¹⁶ based on the AS/ND-LCSS gene expression markers (the pink bar indicates the AS-LCSS genes that are clustered with ASCL1, whereas the blue bar indicates the ND-LCSS genes that are clustered with NEUROD1). d–f Expression levels (FPKM) of ASCL1 (d), IGFBP5 (e), and B4GALT1 (f) in ASCL1^High (n = 54) and ASCL1^Low (n = 25) SCLC patient samples¹⁶. g–i Expression of ASCL1 (g), IGFBP5 (h), and B4GALT1 (i) in ASCL1^High (n = 12) and ASCL1^Low (n = 11) SCLC patient samples³³. MAS5 intensity value averages of all probes for each gene in ASCL1^High and ASCL1^Low samples are indicated. Unpaired, two-tailed, Welch correction t-test results are indicated alongside the violin plots. The horizontal lines indicate the median and quartiles. Source data are provided as a Source Data file