Table 1 Enzyme kinetic parameters for the single sugar transfer of UGT76G1

From: Hydrophobic recognition allows the glycosyltransferase UGT76G1 to catalyze its substrate in two orientations

 

UGT76G1

 

donor

R1 acceptor

R2 acceptor

 

UDPG

STB

ST

Reb E

Rubu

Reb D

Reb A

Km (μM)

5.6 ± 0.4

4.8 ± 0.4

25.5 ± 3.4

9.2 ± 0.7

54.4 ± 4.0

94.2 ± 15.5

243.4 ± 28.1

kcat (min−1)

4.1 ± 0.1

4.8 ± 0.1

41.8 ± 1.4

21.3 ± 0.5

5.9 ± 0.1

3.3 ± 0.2

0.12 ± 0.004

kcat/Km (S−1 mM−1)

12.4

16.5

27.3

38.6

1.8

0.6

0.008

  1. Source data are provided as a Source Data file