Fig. 2
From: Patient-derived pancreas-on-a-chip to model cystic fibrosis-related disorders
Characterization of pancreatic ductal epithelial cells. (a–f, l: Characterization of organoids). a Characterization of pancreatic ductal organoids using epithelial cell markers, b cytokeratin 19 (KRT 19, c E-cadherin, e sodium transport channel (ENaC), and f ZO-1. d Hematoxylin and eosin (H&E) image shows the orientation of pancreatic ductal epithelial cells in spheroid of the organoid. (g–k: Characterization of monolayer of pancreatic ductal epithelial cells (PDECs)). g Phase contrast and j H&E images show monolayer of PDECs formed from the organoids. Monolayer of PDECs showed positivity for tight junction h ZO-1, i F-actin and KRT 19, and k cystic fibrosis transmembrane conductance regulator (CFTR). l RNA-sequencing data was obtained from the pancreatic ductal organoids and verified the PDEC origin (n = 4 sample preparation from the same patient). Data are mean ± SD. Scale bars: 10 µm (k), 20 µm (b, c, e, f, h–j enlarge), 50 µm (d, g, j), and 500 µm (a)
