Fig. 3
From: Patient-derived pancreas-on-a-chip to model cystic fibrosis-related disorders
Monitoring cystic fibrosis transmembrane conductance regulator (CFTR) channel function and endocrine function. CFTR channel function was monitored by stimulating cAMP with forskolin (FSK) using a fluid secretion assay for pancreatic ductal organoids (n > 450 organoids; from 21 pancreatitis patients; data are mean ± SE) and b short-circuit current measurement in polarized monolayer of ductal epithelial cells grown on a trans-well filter. CFTR channel is activated by FSK and inhibited by CFTRinh−172. c Phase contrast image shows cultured pancreatic islet in vitro. d Pancreatic islets were examined by immunofluorescence detection of insulin (green) and glucagon (red). e Endocrine function was monitored by incubating pancreatic islets with different concentrations of glucose-containing media (100 and 450 mg/dL) for 1 h serially. Pancreatic islets were stimulated by high glucose (n = 3 sample preparation from the same patient; data are mean ± SD). Scale bars: 100 µm (c) and 20 µm (d). (p values from one-way analysis of variance (ANOVA) and adjust using Bonferroni factor: *<0.01, **<0.005, ****<1.0 × 10−20)
