Fig. 3

rEV can be detected and quantified using fluorescence-, protein- and RNA-based assays. rEV can be directly quantified using fluorescent NTA (fNTA) and fluorescence triggered high-resolution flow cytometry (HR-FC). a Size distributions under both scatter and fluorescence NTA mode and b the percentage of fluorescent rEV (ratio fNTA/NTA) detected above detection threshold (n = 29). c Linear correlation analysis of ½ dilutions of rEV in PBS measured by fNTA (n = 3). d Scatter plots showing quantification of rEV with HR-FC (n = 3). e Relative concentrations of rEV directly quantified by fNTA versus HR-FC (n = 3). rEV can be indirectly quantified using fluorescent plate reader, p24 ELISA, western blot and RT-qPCR. f Linear correlation analysis of relative fluorescence units (RFU) measured by a fluorescent plate reader versus number of rEV (n = 3). g Linear correlation analysis of p24 concentration measured by a p24 ELISA assay versus number of rEV (n = 3). h Western blot analysis for EGFP on the maximum detectable concentration range with i linear correlation analysis of protein band intensity versus rEV number. j Semi-logarithmic correlation analysis of EGFP mRNA Cq values calculated by RT-qPCR versus number of rEV (n = 3) (measurements in c, f, g, j are performed in triplicate and presented as (mean, SD)). Source data are provided as a source data file