Fig. 2 | Nature Communications

Fig. 2

From: Highly branched  poly(β-amino ester) delivery of minicircle DNA for transfection of neurodegenerative disease related cells

Fig. 2

Biophysical characterization of HPAEs differing in branched structure. a All the polymers have strong and similar DNA binding affinity. b All the polyplexes have sizes < 250 nm and LPAE/DNA polyplexes have a smaller size in comparison with HPAE/DNA counterparts. c As the branching degree increases, the zeta potential of polyplexes increases and reaches at a stable level at 12 mV. d LPAE/DNA and HPAE-3/DNA polyplexes manifest substantially different morphologies, the former is spherical while the latter is toroidal, the scale bars represent 100 nm. e HPAE-3/DNA polyplexes show much stronger cellular uptake and endo/lysosomal escape efficiency, compared to the LPAE/DNA counterparts. The scale bars represent 20 μm. Data are shown as average ± SD; n = 4

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