Fig. 5 | Nature Communications

Fig. 5

From: Highly branched  poly(β-amino ester) delivery of minicircle DNA for transfection of neurodegenerative disease related cells

Fig. 5

Disulfide-containing HPAESSs show higher transfection efficacy than HPAE-3 and commercial reagents. a, b Gluciferase activity of HKC8 and HeLa cells after transfection with different vectors. Data points marked with asterisks (*) are statistically significantly higher relative to the HPAE-3 group. c Fluorescence images of HeLa cells 48 h post transfection with various vectors at the w/w ratio of 20:1. The scale bars represent 200 μm. d The percentage of GFP-positive cells after transfection, asterisk indicates significantly higher transfection efficiency in comparison with the HPAE-3 group. e Representative histogram distributions of UT HeLa cells and the ones after transfection with HPAESSs at the w/w = 20:1. Data are shown as average ± SD; n = 4

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