Fig. 6 | Nature Communications

Fig. 6

From: Highly branched  poly(β-amino ester) delivery of minicircle DNA for transfection of neurodegenerative disease related cells

Fig. 6

Improvement of polyplex cellular uptake by guanidinylation of HPAESSs. a TEM images show that HPAESS-4/DNA and HPAESG-1/DNA polyplexes have different morphologies, the former is uniform and toroidal while the latter is polyhedral, the scale bars present 500 nm. b Fluorescence images of HeLa cells after incubation with HPAESS-4/DNA and HPAESG-1/DNA polyplexes for 4 h at the w/w ratio of 20:1, the latter clearly show higher cellular uptake efficiency, the scale bars present 20 μm. c The cellular uptake efficiency of different HPAESG/DNA polyplexes showing similar percentages of Cy3-positive HeLa cells. d Cells after treatment with HPAESG/DNA polyplexes have higher relative fluorescence intensity than the HPAESS/DNA counterparts. Data are shown as average ± SD; n = 4

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