Fig. 9 | Nature Communications

Fig. 9

From: Highly branched  poly(β-amino ester) delivery of minicircle DNA for transfection of neurodegenerative disease related cells

Fig. 9

Construction of NGF encoding plasmids and evaluation of transfection efficacy. a Production of MC NGF in ZYCY10P3S2T E. coli. MC are produced from Parental plasmid using PhiC31 Integrase system. b GFP expression of Neu7 after transfection with HPAESG-1/PP NGF and HPAESG-1/MC NGF at the w/w = 20:1. The scale bars represent 200 μm. c Representative histogram distributions of UT Neu7 and the ones after transfection with HPAESG-1/PP NGF and HPAESG-1/MC NGF at the w/w = 20:1. d Human beta-NGF concentration of supernatants from Neu7 astrocytes after transfection with HPAESG-1/PP NGF or HPAESG-1/MC NGF at the w/w = 20:1. Data are shown as average ± SD; n = 2

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