Fig. 1

Dynamic integration of newly generated hippocampal neurons in the neurogenic zone. a Experimental timeline of retroviral birth dating (top) and representative image of retrovirally birth-dated GFP⁺ newborn DGCs at 5 dpi (bottom). Scale bar is 15 μm. b Schematic of the measured angle of the leading process of a given DGC with respect to the SGZ (left). Percentages of GFP⁺ newborn DGCs with angles of the leading processes between −10° and 10° over time (right, n = 3–4 mice). c Diagram of in vivo deep brain imaging of newborn DGCs in the SGZ. d Representative images of migrating cells over time in vivo, taken from Supplementary Movie 1. Scale bar is 50 μm. e Schematic of dispersion (top left) and measurements of absolute distance, displacement (final−initial), and speed of imaged GFP⁺ DGCs during the recorded time window (n = 3 mice). f Diagram of adult hippocampal slice culture imaging of newborn DGCs in the SGZ. g Representative images of migrating cells in adult hippocampal slices culture over time, taken from Supplementary Movie 3. Scale bar is 20 μm. h Distributions of migration (absolute) distance and average speed of newborn DGCs in the SGZ and the percentages of leapfrog-like dispersing cells from all the observed newborn cells in adult hippocampal slice culture (n = 3 mice)