Fig. 4

Methylation-, count- and size-based analyses of plasma EBV DNA in the validation sample set. The cutoffs in the corresponding analyses defined in the exploratory dataset are shown. a The EBV DNA methylation scores of the NPC patients (from both the screening and external cohorts) and non-NPC subjects with transiently positive and persistently positive results are shown. The same cutoff value of 73.7 defined in the exploratory dataset is denoted by the red dotted line. b The proportion of EBV DNA reads among the total number of sequenced plasma DNA reads (both human and viral) of the NPC patients (from both the screening and external cohorts) and non-NPC subjects with transiently positive and persistently positive results are shown. The same cutoff value of 2.7 × 10⁻⁵ defined in the exploratory dataset is denoted by the red dotted line. c The EBV DNA size ratios of the NPC patients (from both the screening and external cohorts) and non-NPC subjects with transiently positive and persistently positive results are shown. The same cutoff value of 5.0 defined in the exploratory dataset is denoted by the red dotted line. Source data are provided as a Source Data file