Fig. 3
EV-D68-947 is acid-independent and has altered ICAM-5-binding properties. a HeLa-R19 cells were treated with 200 nM bafilomycin A1 (BafA1), infected and stained for dsRNA, followed by quantification of infected cells. The experiment was conducted twice with similar results. b Viruses were incubated at 48 °C for the indicated times, followed by infection of HeLa-R19 cells, dsRNA staining and quantification of infected cells. Four technical replicates with a fitted sigmoidal curve are shown. c Viruses were incubated with soluble ICAM-5 (sICAM-5), followed by infection of HeLa-R19 cells, dsRNA staining and quantification of infected cells. The experiment was conducted twice with similar results. d WT or ICAM-5KO HAP1 cells were infected and stained for dsRNA, followed by quantification of infected cells. Error bars represent the mean ± s.e.m. of three (a, b) or four (c, d) technical replicates
